Marker-assisted Selection of Bivoltine Silkworm Genetic Resources for Thermotolerance

The aim of the study is identification bivoltine breeds showing thermotolerance to evaluate for regional abiotic stress conditions achieve successful cocoon production.
 Study Design: Lepidopteran insect, Bombyx mori silkworm more prone crop losses due hot climatic during summer seasons. In order produce cocoons consistently throughout year without affecting productivity season, high temperature tolerant are indispensable. vast B.mori (L.) germplasm available needs be screened using reliable molecular markers select efficient productive with tolerance.
 Marker assisted selection (MAS) has been widely used in desired traits breeding programs. It believed that based on trait linked DNA marker would better option than phenotypic which laborious and time consuming.
 Place Duration Study: taken up 3 years collaboration Seribiotech Research Laboratory, Kodathi
 Methodology: present carried out screen forty selected B. resources exhibiting higher rate survival SSR (LFL329; LFL1123; S0809; S0813) so as identify thermotolerant breeds.
 Results: Based PCR product resolved 2% agarose gel presence bands corresponding thermosusceptible observed, 10 accessions showed 87-100% were viz. BBI-0086 (KPG-A), BBE-0184 (SMGS-2), BBI-0301 (YS-7) BBI-0339 (DD-2) spinning dumbbell cocoons- BBI-0044 (NB4D2), BBI-0334 (APS-4), BBI-0336 (APS-8), BBI-0338 (DD-1), BBI-0343 (NK3) BBI-0358 (CSR26) further validation through field trials hotspots Tropical - High low humidity at RSRS, Jammu REC Chitradurga; Sub tropical RSRS Temperate CSRTI, Berhampore.
 Conclusion: elucidate impact associated over economic performances accessions. findings provide a suitable platform future improvement program.